Standardized Micro-Scale Mixing Evaluation

The field of microfluidics has become increasingly important with the development of micro-fuel cells, DNA arrays, and various advancements towards the labon-a-chip concept. While researchers have proposed many methods of mixing, there has been no standardized comparison on the mixing efficiency of the proposed methods in literature. In this paper, the students implemented an image-based technique for evaluating the standardized mixing of results from a previous experiment. The results were then used as experimental validations of corresponding simulations. An additional program was developed to allow future researchers to evaluate mixing in any userdefined area of an image.